ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of Xiezhuo Chubi Decoction (XZCBD) on the microRNA expression patterns of kidney in mice with hyperuricemia.</p><p><b>METHODS</b>Sixty Kunming male mice were randomly divided into the high-, medium-, and low-dose XZCBD groups, benzbromarone group, model group, and control group. Except the control group, all mice were established with yeast method combined with uricase inhibition method to build hyperuricemia model, and the corresponding drugs (37.5 g/kg, 18.75 g/kg, 9.375 g/kg, and 0.02 g/kg per day) were administrated on the 7th day. On the 22nd day, the blood uric acid concentration was detected, and microRNA with obvious changes in kidney was screened with qRT-PCR.</p><p><b>RESULTS</b>The uric acid in the model group was higher than that in the control group, and the levels of the uric acid were reduced after being treated with XZCBD; the differences among groups were significant (P<0.05). Compared with the control group, 32 kinds of microRNA expression changes were detected on the 15th day after being treated with high-dose XZCBD by microRNA expression profile screening. Among them, miR-34a could inhibit the expression of human urate anion exthanger 1, and miR-146a might have inhibited the inflammatory reaction.</p><p><b>CONCLUSION</b>XZCBD could significantly reduce the serum uric acid level; its effect on hyperuricemia might be through affecting microRNA expressions.</p>
Subject(s)
Animals , Male , Mice , Base Sequence , Dose-Response Relationship, Drug , Drugs, Chinese Herbal , Pharmacology , Therapeutic Uses , Gene Expression Profiling , Gene Expression Regulation , Hyperuricemia , Blood , Drug Therapy , Genetics , Kidney , Metabolism , Pathology , MicroRNAs , Genetics , Molecular Sequence Data , Organic Anion Transporters , Genetics , Metabolism , Reverse Transcriptase Polymerase Chain Reaction , Uric Acid , BloodABSTRACT
Objective:To produce rhBMP-4 with bioactivity in E.coli. Methods: The full-length human BMP-4 gene was mutated by PCR without changes in amino acid sequence, then the synthesized gene was cloned into plasmid pET-3c, transducted into BL21(DE)plysS, and induced by adding IPTG to a final concentration of 1.0 mmol/L. The protein product was purified using ion-exchange chromatography method and then renaturated, bioactivity was checked by C2C12 differentiation in vitro and mouse ectopic bone formation in vivo. Results: A 438 bp gene fragment encoding mature peptide of hBMP-4 was cloned , the protein product was mostly in the form of inclusion body, after renaturation, the engineering protein shows better bioactivity. Conclusion:The mutant strategy can enhance the expression of bioactive rhBMP-4 in E.coli expression system.
ABSTRACT
<p><b>OBJECTIVE</b>To study the relationship between aberrant FHIT transcripts and hepatocellular carcinoma (HCC).</p><p><b>METHODS</b>Reverse transcription-polymerase chain reaction (RT-PCR) and single strand conformational polymorphism (SSCP) assays were used to analyze the transcripts and mutations of FHIT gene in 24 matched tumorous tissues and para-tumorous tissues from patients with HCC and in 4 normal liver tissues.</p><p><b>RESULTS</b>Aberrant FHIT transcripts were observed in 11 out of 24 (46%) tumorous tissues and in 2 (8%) of the matched para-tumorous tissues.</p><p><b>CONCLUSION</b>FHIT aberrant transcripts may play an important role in the pathogenesis of hepatocellular carcinoma.</p>